open access article

Navarro D., Chaduli D., Taussac, S., Lesage-Meessen L., Sacha Grisel, Haon M., Callac P., Courtecuisse R., Decock C., Dupont J., Richard-Forget F., Fournier J., Guinberteau J., Lechat C., Moreau P.-A., Pinson-Gadais L., Rivoire B., Sage L., Welti S., Rosso M.-N., Berrin J.G., Bissaro B., Favel A. (2021) Large-scale phenotyping of 1,000 fungal strains for the degradation of non-natural, industrial compounds. Communications Biology 4, 871. https://doi.org/10.1038/s42003-021-02401-w

Abstract

Fungal biotechnology is set to play a keystone role in the emerging bioeconomy, notably to
address pollution issues arising from human activities. Because they preserve biological
diversity, Biological Resource Centres are considered as critical infrastructures to support the
development of biotechnological solutions. Here, we report the first large-scale phenotyping
of more than 1,000 fungal strains with evaluation of their growth and degradation potential
towards five industrial, human-designed and recalcitrant compounds, including two synthetic
dyes, two lignocellulose-derived compounds and a synthetic plastic polymer. We draw a
functional map over the phylogenetic diversity of Basidiomycota and Ascomycota, to guide the
selection of fungal taxa to be tested for dedicated biotechnological applications. We evidence
a functional diversity at all taxonomic ranks, including between strains of a same species.
Beyond demonstrating the tremendous potential of filamentous fungi, our results pave the
avenue for further functional exploration to solve the ever-growing issue of ecosystems
pollution